Immunophenotyping

Author(s): S. Fruehauf, A. Pitkus
Institution(s): Heidelberg University Medical Centre
Last change of this image collection: 2004/08/19

Immunophenotyping: Immunophenotyping was performed on a fresh bone marrow specimen. In the forward and side scatter areas, the mononuclear cell populations are not well defined. In the CD45 side scatter area is a population comprising 18 % of the total cells with a low CD45 expression and low side scatter properties. A population with these same characterstics is also present in 60% of the cells in the monocytoid gate and 14% of the cells in the lymphocytoid gate. The lymphoblastoid gate contains a population of immature myeloid cells comprising 20 to 30 % of the total cells with the following phenotype: CD34+, CD33+, CD13+, CD2+, HLA-DR+, CD11b+/-, CD14-, CD15-/+, CD117+, CD19-, and CD10-. In the lymphoblastoid gate, there is an additional population of monocytes comprising 59% of cells.

Comment on this image collection: The immunophenotyping results are consistent with the transition of a CMML to an HLA-DR positive acute myeloid leukemia wih an aberrant expression of the T-cell marker, CD2.