In the blood differential of the SYSMEX X-CLASS leukocytes are classified according to their content of nucleic acids (fluorescence intensity) and the complexity of their internal structure (side scatter). In the scattergram of a healthy individual the different cell populations are clearly separated: lymphocytes (LYMPH), monocytes (MONO), neutrophil and basophil granulocytes (NEUT+BASO) as well as eosinophilic granulocytes (EO). The basophil granulocytes are counted as a separate population in a different channel (WBC/BASO channel, not shown). The number of neutrophil granulocytes is calculated by subtracting the basophil granulocytes from the 'NEUT+BASO' count.
On the haematology analysers SYSMEX XE-5000, XE-2100, XT-2000i and XT-1800i also the population of immature granulocytes (IG) can be counted and displayed separately (see month 09, figure 1). For the XE-2100, XT-2000i and XT-1800i a dedicated software (IG MASTER) is required for this. On the XE-5000 this feature is integrated.